https://pages.htgaa.org/2026b/wanqi-yuki-fang/week3-cell-free/index.htmlA. COMPARE CELL-FREE REACTION COMPOSITIONS (20 minutes) Why does the concentration of potassium glutamate and magnesium glutamate differ between compositions? Which is more sensitive? The concentrations of potassium glutamate and magnesium glutamate differ between formulations because CFPS performance depends on the chemical environment, so reagent concentrations need to be optimized together. Magnesium appears to be more sensitive because its concentration may need to be re-optimized for different lysate batches, and the reading also notes that protein synthesis depends nonlinearly on free Mg²⁺ and nucleotide concentrations.Hugoen