https://pages.htgaa.org/2026b/xavier-lewis-palmer/week-01-hw-project-ideas/index.htmlProject ZP Freeze (1) What you would like to biomanufacture and why? I would like to biomanufacture freezing responsive antifreeze proteins like Fr10 and Li16. I am interested in their use in helping to cryopreserve frogs, as well as their (or similar material) potential in helping to preserve animal tissues and organs. As a first stage, I merely wish to create more of them and similar. Source: Biggar KK, Kotani E, Furusawa T, Storey KB. Expression of freeze-responsive proteins, Fr10 and Li16, from freeze-tolerant frogs enhances freezing survival of BmN insect cells. FASEB J. 2013 Aug;27(8):3376-83. doi: 10.1096/fj.13-230573. Epub 2013 May 8. PMID: 23657819. https://pubmed.ncbi.nlm.nih.gov/23657819/ Cai, Q. and Storey, K.B. (1997) ‘Upregulation of a novel gene by freezing exposure in the freeze-tolerant wood frog (Rana sylvatica)’, Gene, 198(1–2), pp. 305–312. Available at: https://doi.org/10.1016/S0378-1119(97)00332-6. McNally, J.D., Wu, S.B., Sturgeon, C.M. and Storey, K.B. (2002) ‘Identification and characterization of a novel freezing-inducible gene, li16, in the wood frog Rana sylvatica’, The FASEB Journal, 16(8), pp. 902–904. Available at: https://doi.org/10.1096/fj.02-0017fje. Le Tri, D., Childers, C.L., Adam, M.K., Ben, R.N., Storey, K.B. and Biggar, K.K. (2019) ‘Characterization of ice recrystallization inhibition activity in the novel freeze-responsive protein Fr10 from freeze-tolerant wood frogs, Rana sylvatica’, Journal of Thermal Biology, 84, pp. 426–430. Available at: https://doi.org/10.1016/j.jtherbio.2019.07.030.Hugoen