https://pages.htgaa.org/2026b/xiangtao-shi/week-03-hw-cell-free/index.htmlA. Compare cell-free reaction compositions Potassium glutamate and magnesium glutamate Why do their concentrations differ between compositions? Which is more sensitive? Potassium glutamate contributes to the ionic environment and supplies glutamate. Potassium is essential for translation. Glutamate replaces chloride as the counter-ion because chloride may inhibit some CFPS enzymes. Magnesium glutamate supplies Mg²⁺ for ribosomes, nucleic-acid interactions and enzyme activity. Magnesium holds ribosomes together and keeps tRNAs folded correctly. Potassium glutamate and magnesium glutamate concentrations differ between compositions because they have different functions and their optimal levels depend on the whole reaction formulation.Hugoen