https://pages.htgaa.org/2026b/zia-baig/week-03-hw-cell-free-reaction-design/index.htmlZia Baig | HTBAA 2026 | October 6, 2026 A. Comparing reaction components Potassium glutamate helps maintain the ionic conditions needed for protein synthesis. Magnesium glutamate supports ribosomes and many enzymes, but nucleotides and phosphate bind magnesium and change how much remains available. Magnesium is therefore the more sensitive variable: small changes can sharply reduce expression, while potassium usually has a broader useful range (Olsen et al., 2026).Hugoen