{
  "candidate_aa": 90,
  "candidate_cysteines": 0,
  "tag_and_linker_aa": 10,
  "expressed_aa": 100,
  "cds_bp_including_stop": 303,
  "backbone_original_bp": 5369,
  "replaced_segment_1based": [
    88,
    250
  ],
  "replaced_bp": 163,
  "final_plasmid_bp": 5509,
  "synthetic_fragment_bp_including_overlaps": 363,
  "retained_backbone_bp": 5206,
  "codons_changed": 62,
  "bases_changed": 67,
  "before": {
    "gc_percent": 50.33,
    "CAI_usage_table": 0.5828,
    "longest_homopolymer": 5,
    "gc_50bp_min_percent": 36,
    "gc_50bp_max_percent": 64
  },
  "after": {
    "gc_percent": 52.33,
    "CAI_usage_table": 1.0,
    "longest_homopolymer": 6,
    "gc_50bp_min_percent": 38,
    "gc_50bp_max_percent": 68
  },
  "translation_exact": true,
  "no_internal_stops": true,
  "one_terminal_stop": true,
  "all_DNAChisel_constraints_pass": true,
  "unchanged_backbone_outside_replacement": true,
  "overlap_assembly_reconstructs_final_circle": true,
  "biological_expression_or_binding_tested": false,
  "external_similarity_search": "pending permission / not run",
  "biosecurity_screen": "not run; no screening access configured",
  "cds": "ATGCATCATCATCATCATCATGGCAGCGGCGATGAAAAAGCGAAAGAACTGCTGAAAAAAGCGAAAGAAGCGGGCAAAAAAGCGCTGGAAGAAGTGAAAAAAGCGAAAGAAAAAGGCGATCTGGGCGCGGCGAGCAAAGCGGCGGCGGAAGGCACCGAAAAAATGGGCGAACTGGTGCAGGAAGCGGTGGATCTGGGCCTGCCGCAGGAAGCGATTGATGAACTGAAAAAACTGCTGTTTGAAACCGGCCGCGAAATGATGAAACTGGTGGAAGAAACCCGCGAAGCGCGCGAAAAAGCGTAA",
  "baseline_cds": "ATGCATCATCATCACCACCACGGTTCGGGAGATGAGAAAGCAAAGGAACTACTGAAGAAAGCAAAGGAGGCGGGGAAGAAAGCGCTTGAAGAAGTAAAGAAAGCAAAAGAAAAGGGTGACTTAGGTGCAGCATCGAAGGCCGCAGCTGAAGGCACAGAAAAGATGGGAGAACTCGTCCAAGAGGCGGTCGACCTGGGGCTCCCTCAAGAAGCCATTGACGAACTTAAAAAGTTGCTTTTTGAAACGGGGCGCGAAATGATGAAACTTGTTGAGGAGACTCGCGAAGCCCGGGAGAAAGCCTAA",
  "protein": "MHHHHHHGSGDEKAKELLKKAKEAGKKALEEVKKAKEKGDLGAASKAAAEGTEKMGELVQEAVDLGLPQEAIDELKKLLFETGREMMKLVEETREAREKA",
  "candidate": "DEKAKELLKKAKEAGKKALEEVKKAKEKGDLGAASKAAAEGTEKMGELVQEAVDLGLPQEAIDELKKLLFETGREMMKLVEETREAREKA",
  "left_overlap": "TTTTGTTTAACTTTAAGAAGGAGATATACC",
  "right_overlap": "GATCCGGCTGCTAACAAAGCCCGAAAGGAA"
}